Generation of induced pluripotent stem cell line from foreskin fibroblasts
نویسندگان
چکیده
منابع مشابه
Generation of human induced pluripotent stem cells from dermal fibroblasts.
The generation of patient-specific pluripotent stem cells has the potential to accelerate the implementation of stem cells for clinical treatment of degenerative diseases. Technologies including somatic cell nuclear transfer and cell fusion might generate such cells but are hindered by issues that might prevent them from being used clinically. Here, we describe methods to use dermal fibroblasts...
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Recent studies have demonstrated that embryonic stem cell-like induced pluripotent stem (iPS) cells can be generated by enforced expression of defined transcription factors. The fact that cell fate change is accompanied by changes in epigenetic modifications prompted us to investigate whether chemicals known to modulate epigenetic regulators are capable of enhancing the efficiency of iPS cell g...
متن کاملEnhanced Generation of Induced Pluripotent Stem Cells from a Subpopulation of Human Fibroblasts
BACKGROUND The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming. METHODOLOGY/PRINCIPAL FINDINGS Here, we report identification and isolation of a subpopulation of human dermal...
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Induced pluripotent stem (iPS) cells can be generated from somatic cells by transduction with several transcription factors in mouse and human. However, direct reprogramming in other species has not been reported. Here, we generated monkey iPS cells by retrovirus-mediated introduction of monkey transcription factors OCT4, SOX2, KLF4, and c-MYC.
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Dear Editor, Somatic cells can be reprogrammed into pluripotent stem cells, called induced pluripotent stem cells (iPSCs), by defined transcription factors (Takahashi et al., 2006). The reprogramming of somatic cells may be a continuous stochastic process in which nearly all somatic donor cells have the ability to give rise to iPSCs with continuous passaging and the expression of defined factor...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2016
ISSN: 1873-5061
DOI: 10.1016/j.scr.2016.09.014